Extended Data Fig. 11: Endothelial cells as a discovery model for evaluating MYCT1 moderated signalling responses.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a,b Evaluating the effects of MYCT1 KD on endocytosis. Quantification and representative FACS histogram of fluorescent low molecular weight (10 KDa) dextran internalized over 30 min in control or MYCT1 KD E4ECs (a) or non-immortalized HUVECs (b) 72 h after transduction. For E4EC n = 10 (ctrl), n = 8 (KD1) and n = 4 (KD2) from 3 independent experiments, for HUVEC n = 4 replicates from 3 independent experiments, mean±s.e.m, two-tailed paired t-test. c-g Phosphoproteomic analysis of the effects of MYCT1 KD in E4EC 72 h after transduction from n = 1 experiment in technical duplicates. c Experimental outline. d Heatmap depicting the Log2FC for all identified phosphorylated protein sites. Pearson correlation between the two different MYCT1 KD vectors calculated using Excel is included. e Functional enrichment analysis of the differentially phosphorylated proteins. LogP values for selected KEGG, Reactome (REAC) and WikiPathway (WP) terms for the proteins with increased (red) or decreased (blue) phosphorylation after MYCT1 KD compared to control. Performed using g:Profiler69 with g:SCS multiple testing correction method applying significance threshold of 0.05. f Predicted relative kinase activity (KSEA59) in MYCT1 KD compared to control. g Predicted pathways and perturbations enriched after MYCT1 KD determined by PTM-SEA56. Schematic in c was created with BioRender (https://www.biorender.com).
