Fig. 2: MYCT1 governs regulatory programmes associated with human HSC functional competence.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a, scRNA-seq dot plot documenting the expression of selected human HSC-enriched genes from multiple datasets18,33,34,35,36, as well as the HSC activation marker CDK6, that are significantly differentially expressed in uncultured CB HSPCs (CD34+CD38–CD90+) selected on the basis of HLF and the presence or absence of MYCT1 expression. b, scRNA-seq dot plot depicting the scores for the differentially regulated functional categories comparing HLF+ HSCs from cultured control, MYCT1 KD and MYCT1 OE HSPCs. Uncultured and cultured HLF+ HSCs are also compared. c, Dot plot depicting gene expression of ETS factors. d, Dot plot depicting gene expression of selected mitochondrial and OXPHOS genes. e,f, Quantification (left) and representative FACS plots (right) of mitochondrial membrane potential (TMRE) (e) and mitochondrial reactive oxygen species (MitoSOX) (f) in control, MYCT1 KD and MYCT1 OE HSPCs 72 h after transduction. Delta median fluorescence intensity (ΔMFI) is between the GFP+ cells and the GFP– cells within the same sample. n = 5 replicates from 3 independent experiments, mean ± s.e.m., two-tailed unpaired t-test.
