Extended Data Fig. 1: Silencing of MYCT1 expression in cultured human HSPCs.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a MYCT1 expression in human sorted hematopoietic populations from the DMAP dataset29,31. b Myct1 expression in mouse hematopoietic populations from Bloodspot31. c-g Relative expression of MYCT1 and other HSC regulatory genes in sorted FL or CB HSPCs that were isolated freshly and after culture in different conditions. c Microarray analysis of CD34+CD38−CD90+FL HSPCs co-cultured with OP9 stroma supplemented with cytokines as indicated9. Mean±s.e.m. of all available microarray probes for MYCT1 (2), MECOM (5), and RUNX1 (10) from n = 3 (uncultured and week 2) or n = 2 (week 5) independent experiments. d RNAseq analysis of CD34+CD38−CD90+CD45RA-CD49f+ CB LT-HSPC cultured without stroma as indicated28. e RT-qPCR of CD34+CD38−CD90+ CB HSPCs co-cultured with E4EC13 as indicated. n = 2 experiments, mean and individual data points. f,g Gene expression (RNAseq) and UCSC genome browser tracks of MYCT1 and MECOM genomic regions showing RNA seq and Chip-seq of histone marks in CD34+CD38−CD90+ FL HSPCs isolated freshly or co-cultured with OP9 stroma as indicated10. Gene expression is from n = 3 (uncultured) or n = 2 (week 4) experiments, mean with individual data points.
