Extended Data Fig. 6: Genome-wide binding data from ChIP-seq experiments suggest a high mismatch tolerance for some TldR homologs.
From: TnpB homologues exapted from transposons are RNA-guided transcription factors
a, Genome-wide ChIP–seq profiles for the indicated fliCP-associated TldR homologs, normalized to the highest peak within each dataset. The magnified insets at the bottom show the off-target sequences (grey) compared to the intended (engineered) on-target sequence (purple), with TAMs in yellow. Off-target #3 has no clear TAM-flanked off-target sequence but is intriguingly located at a tRNA locus, and binding was observed for diverse fliCP- and oppF-associated TldRs that recognized distinct TAMs. The phylogenetic tree at right indicates the relatedness of the tested and labeled homologs. b, Results for the indicated oppF-associated TldR homologs, shown as in a.
