Extended Data Fig. 7: Plasmid interference assays confirm that TldR homologs lack detectable nuclease activity.
From: TnpB homologues exapted from transposons are RNA-guided transcription factors
a, Schematic of E. coli-based plasmid interference assay using pEffector and pTarget. b, Representative dilution spot assays for GstTnpB3 and synthetically inactivated RuvC mutant (D196A), showing the entire plate (left) and the magnified area of plating. Transformants were serially diluted, plated on selective media, and cultured at 37 °C for 16 h. Colony visibility was enhanced by inverting the colors and increasing contrast/brightness. c, Dilution spot assays for the indicated fliC-associated TldR homologs (left) and closely related TnpB homologs (right). Non-targeting (NT) gRNA controls are shown at the bottom, and the phylogenetic tree indicates the relatedness of the tested proteins. d, Results for the indicated oppF-associated TldR and TnpB homologs, shown as in c.
