Extended Data Fig. 9: Enterobacter RNA-seq data confirm the native expression of gRNAs from fliCP-tldR loci.
From: TnpB homologues exapted from transposons are RNA-guided transcription factors
a, RNA-seq read coverage from three Enterobacter strains that natively encode fliCP-tldR loci, revealing clear peaks associated with mature gRNAs containing ~95–97-nt scaffolds and 16-nt guides. Data from three biological replicates are overlaid. b, Predicted secondary structure and sequence of the gRNA associated with EhoTldR. c, Multiple sequence alignment of the DNA encoding gRNA scaffold sequences for representative fliCP-associated TldRs, with conserved positions colored in darker blue.
