Extended Data Fig. 3: RIP-seq reveals that some oppF-associated TldR proteins use short, 9–11-nt guides.
From: TnpB homologues exapted from transposons are RNA-guided transcription factors
a, RNA immunoprecipitation sequencing (RIP-seq) data for an oppF-associated TldR homolog from Enterococcus faecalis (Efa1TldR) reveals the boundaries of a mature gRNA containing a 9-nt guide sequence. Reads were mapped to the TldR-gRNA expression plasmid; an input control is shown. b, Published RNA-seq data for Enterococcus faecalis V583 reveals similar gRNA boundaries, including an approximately 11-nt guide. c, RIP-seq data as in a for a second biological replicate of Efa1TldR, further corroborating the observed 9–11-nt guide length.
