Fig. 1: Cryo-ET of glutamatergic ISVs.
From: Structure and topography of the synaptic V-ATPase–synaptophysin complex
a, Schematic overview of glutamatergic synaptic vesicle isolation and cryo-EM sample preparation (Methods). LP2, second lysis pellet. Schematic overview in a was created using BioRender (https://biorender.com). b, A representative tomogram (1 of 52 tomograms) (cryoCARE denoised) of ISVs is shown as a tomographic slice (left; thickness 1 nm) with a corresponding 3D rendering (right; Methods). The ISV membrane, intact and V0-only V-ATPase assemblies are coloured in grey, yellow and cyan, respectively. Three representative ISVs are boxed and annotated with the copy numbers of intact and V0-only V-ATPase assemblies. Scale bar, 50 nm. c,d, Subtomogram averaging maps (Methods and Extended Data Fig. 1) of intact (c; state 3) and V0-only (d) V-ATPase assemblies (transparent surfaces). The extra binding partner density (not part of the V-ATPase assembly) is coloured in orange and denoted with an asterisk. Scale bars, 5 nm.
