Fig. 4: Increase of V-ATPase copy numbers and seizures in Syp−/− mice.
From: Structure and topography of the synaptic V-ATPase–synaptophysin complex
a, A representative tomogram (1 of 78 tomograms; cryoCARE denoised) of ISVs from Syp−/− mouse brains is shown as a tomographic slice (left; thickness of 35.5 nm) with a corresponding 3D rendering (right). The ISV membrane, and the intact and V0-only V-ATPase assemblies are coloured in grey, yellow and cyan, respectively. Five representative ISVs are boxed and labelled, with the copy number of intact and V0-only V-ATPase assemblies identified within each. More examples and views are shown in Extended Data Fig. 8a,b. Scale bar, 50 nm. b,c, Distribution of intact (b) and V0-only (c) V-ATPase copy number per wild-type ISV or Syp−/− ISV. Statistical significance tests are shown in Extended Data Fig. 8d,e, and numerical data are available in Supplementary Tables 4 and 5. d, Mice of 4–6 months of age of two genotypes (wild-type (n = 9) and Syp−/− (n = 5)) were injected with kainic acid (25 mg kg−1 intraperitoneally) and observed for 60 min with video recording. Seizure severity was scored blind by two observers using a modified Racine scale, and the latency survival to R6 or R8 seizure severity is reported (Methods). **P = 0.0013 and ***P < 0.0001 (one-sided Gehan–Breslow–Wilcoxon test).
