Extended Data Fig. 2: Single-particle averaging workflow of V-ATPase assemblies in wild-type ISVs (related to Fig. 3).
From: Structure and topography of the synaptic V-ATPase–synaptophysin complex
a, Representative micrograph (one of 21,577 micrographs) of ISVs and V-ATPase assemblies 2D classification averages. The micrograph was Topaz-denoised for better visualization. b&c, Cryo-EM image processing workflow of (b) intact V-ATPase assemblies in three rotational states and (c) V0-only V-ATPase assemblies (see Methods). d&e, Gold-standard FSC curves of the intact V-ATPase assembly in three rotational states and of the V0-only V-ATPase assembly. f, Refined maps of intact V-ATPase State 3 and V0-only V-ATPase assemblies, colored according to local resolution estimated in cryoSPARC (see Methods). g, Particle orientation distributions of intact V-ATPase assemblies in three rotational states and V0-only V-ATPase assemblies (see Methods). The orientational distributions should adequately sample Fourier space.
