Extended Data Fig. 4: Single-particle averaging workflow of V-ATPase assemblies in Syp−/− ISVs.
From: Structure and topography of the synaptic V-ATPase–synaptophysin complex
a, Representative micrograph (one of 20,027 micrographs) of Syp−/− ISVs and V-ATPase 2D classification averages. The micrograph was Topaz-denoised for better visualization. b&c, Cryo-EM image processing workflow of (b) intact V-ATPase assemblies in three rotational states and (c) V0-only V-ATPase assemblies in Syp−/− ISVs (see Methods). d&e, Gold-standard FSC curves of intact V-ATPase assemblies in three rotational states and V0-only V-ATPase assemblies in Syp−/− ISVs. f, Refined maps of intact V-ATPase State 3 and V0-only V-ATPase assemblies colored according to local resolution estimated in RELION and cryoSPARC (see Methods). g, Orientation distributions for the particles of intact V-ATPase assemblies in three rotational states and V0-only V-ATPase assemblies in Syp−/− ISVs (see Methods).
