Extended Data Fig. 6: Adenosine A2B receptor-mediated signalling maintains synaptic function in the hippocampus.
From: Adenosine signalling to astrocytes coordinates brain metabolism and function
Monitoring the excitatory transmission in the CA3-CA1 pathway (acute hippocampal slices). The graph illustrates the relative changes in the averaged field excitatory postsynaptic potential (fEPSP) slope recorded in the CA1 area in response to stimulation of Schaffer collateral (SC) fibers in the absence and presence of A2B receptor antagonist PSB 603 (10 μM). A2B receptor blockade led to a gradual decline in fEPSP slope; this effect of A2B receptor blockade was prevented by supplemental lactate (5 mM). Analysis was performed for the first fEPSP evoked in a low-frequency pulse train (5 pulses at 20 Hz). Data are presented as individual values and means ± s.e.m. Numbers in parentheses indicate the number of independent slice experiments/number of animals per experimental group. P values, one-way ANOVA followed by Sidak’s post hoc test.
