Extended Data Fig. 8: Sleep/wake architecture under conditions of A2B receptor deletion in astrocytes.
From: Adenosine signalling to astrocytes coordinates brain metabolism and function
(a) Bilateral targeting of hippocampal astrocytes of Adora2bflox/flox or wild-type (WT) mice to express iCre recombinase or reporter protein (tdTomato). Summary data illustrating slow wave activity (0.5-4.0 Hz) and low-frequency slow wave activity (0.5-1.5 Hz) during NREM sleep across the light phase in two control groups of mice and animals with A2B receptor knockdown in hippocampal astrocytes (A2BR-Astro-KD). Data are presented as mean values ± s.e.m.; (b) Summary data illustrating the percentage of time spent in wakefulness, NREM (non-rapid eye movement) sleep, or REM (rapid eye movement) sleep during the 24-hour recordings as well as the number and duration of wake, NREM, and REM sleep episodes in two groups of control mice and A2BR-Astro-KD mice. Sleep/wake architecture and accumulation of sleep pressure were not affected when A2B receptor deletion was limited to the astrocytes of the hippocampus; (c) Summary data illustrating the percentage of time spent in wake, NREM or REM sleep during the 24 h recordings and the number and duration of wake, NREM and REM sleep episodes in Adora2bflox/flox: Aldh1l1Cre- and Adora2bflox/flox: Aldh1l1Cre/ERT2+ mice treated with tamoxifen. Deletion of A2B receptors in brain astrocytes led to fragmentation of sleep and wake during the light (resting) phase, with shorter periods of NREM sleep and increased number of wakefulness events. In panels (b) and (c), data are presented as individual values and means ± s.e.m. Numbers in parentheses indicate the numbers of animals per experimental group. P values, two-tailed unpaired t-test.
