Extended Data Fig. 4: Activation of adenosine A2B receptors stimulates astrocyte glucose metabolism.
From: Adenosine signalling to astrocytes coordinates brain metabolism and function
(a) Representative traces and summary data illustrating ATP- and adenosine-induced changes in astrocyte glycolytic rate, recorded using the fluorescent sensor of glucose FLIP12glu-700μΔ6 (FI ratio Citrine/CFP) in the presence of glucose transporter inhibitor Cytochalasin B (CytB, 20 µM). The slope of the sensor signal decline under conditions of glucose transporter blockade was used to calculate the glycolytic rate. The example also illustrates the response to the removal of extracellular glucose, demonstrating the functionality of the sensor. P values, one-way ANOVA followed by Sidak’s post hoc test; (b) Representative traces and summary data illustrating the effect of A2B receptor agonist Bay 60-6583 on cytosolic NADH-NAD+ redox state (reporting glucose consumption) recorded using Peredox sensor in cultured astrocytes. A2B receptor agonist had no effect on astrocyte glucose consumption in conditions of A2B receptor deletion (A2BR-Astro-KD). P value, two-tailed Student’s t-test. Traces illustrate averaged (mean ± s.e.m.) recordings from several individual cells in a representative experiment. In the box-and-whisker plots, the central dot indicates the mean, the central line indicates the median, the box limits indicate the upper and lower quartiles, and the whiskers extend to 1.5 IQR from the quartiles. Numbers in parentheses indicate the number of individual cells/number of separate cultures, prepared from the same number of animals.
