Extended Data Fig. 2: CD96hi cells are a Th22 cell population.
From: Interferon subverts an AHR–JUN axis to promote CXCL13+ T cells in lupus
a, Example of flow cytometry sorting of CD4+ T cell subsets for bulk RNA-seq analysis. b, PCA plot of bulk RNA-seq profiles of CD4+ T cell subsets sorted from SLE (n = 6) or healthy control (n = 5) donors. Colors indicate cell subsets and shapes indicate clinical group. c, Multi-set Venn diagram of the number of differentially expressed genes between CD96hi cells and indicated CD4+ T cell subsets. d, Expression of IL22 and CXCL13 by qPCR in T cell populations from SLE patients (n = 6), plotted relative to expression in CD96hi cells. IL22 expression p-values from left to right: 0.0063, 0.0075. CXCL13 expression p-values from left to right: 0.0128, 0.0012, 0.0283. e, Flow cytometry detection of IL-22 and IL-17A in PMA/ionomycin-stimulated CD96hi CD4 T cells (left) and quantification of IL-22+ IL-17A+ cells (right) in cell subsets from controls (n = 6). Boxes indicate median bounded by 1st and 3rd quartile; bars indicate min/max. f,g, Flow cytometry quantification of cytokines from PMA/ionomycin stimulated CD4+ T cell subsets sorted from healthy donors (f, n = 5, p = 0.0012 for Th17 versus Tph) and base chemokine receptor expression (g, n = 6-7). p-values for g from left to right, all comparing to CD96hi subset, for CCR6: 0.0156, 0.0156, 0.0156, for CXCR5: 0.0313, 0.0313, 0.0313, for CXCR3: 0.0156, 0.0156, 0.0156. Data for f and g are shown as mean ± S.D. P-values (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) were obtained by ratio paired t-test in d, f, g or by Wilcoxon test in e.
