Extended Data Fig. 4: Effects of chronic AHR modulation in CD4+ T cells.
From: Interferon subverts an AHR–JUN axis to promote CXCL13+ T cells in lupus
a, ELISA data for indicated cytokines in supernatants of memory (top) and naïve (bottom) CD4+ T cells re-stimulated each week for 3 weeks, normalized to DMSO 1 week result for each donor (n = 3-4 donors). b, UMAP of RA synovial T cell clusters and expression of CXCL13. c, UMAP of cells from Fig. 2e mapped to RA synovial T cell UMAP. d, Cluster abundance of in vitro cultured memory CD4+ T cells from Fig. 2e mapped to RA synovial T cell clusters (n = 3). Compared to DMSO condition, from top to bottom, for TGF-β + DMSO p = 0.0092, 0.0023, 0.0275, 0.0323, and for TGF-β + AHRinh P = 0.0188, 0.0023, 0.0323. ANOVA with Holm-Sidak test. e, CXCL13 expression (by fragments per kilobase of transcript per million mapped reads, FPKM) in bulk RNA-seq samples of cell stimulated under indicated conditions (n = 3). f, GSEA enrichment plots of Tph gene signature in naïve or memory CD4+ T cells stimulated with TGF-β plus either AHR agonist TCDD or inhibitor (AHRinh) CH-223191. g, GSEA enrichment plots for Tph gene signature in T cells stimulated with or without TGF-β, under indicated conditions of AHR agonist TCDD, AHR inhibitor (AHRinh) CH223191, or DMSO control. Mean ± SD shown in a, e.
