Extended Data Fig. 4: Increased ocSSCs from Control Bones After Sharing Circulation with Mutant Esr1Nkx2.1-Cre Females, who Exhibit Youthful ocSSCs and Bone Mass.
a, Box and whisker plots of live cells obtained following FACS-purification as described in Methods isolated from control femurs obtained from WT:WT or WT:MUT parabionts (N = 6, 4) or from control female (red, N = 6, 7) or male (blue, N = 4, 4) femurs transplanted into Esr1Nkx2.1-Cre females as indicted by legend on top. b, Bar graphs of CFU-F from ocSSCs purified from control or mutant female long bones at age indicated (N = 3–6). c, Functional in vitro assays of ocSSCs from 54-week-old females in defined media and stained with dye as indicated at the top of representative images of culture wells (n = 3-4 replicates); Crystal Violet (CFU-F), Alizarin Red (osteogenesis), and Alcian Blue (chondrogenesis). d, Fractional bone volume of trabecular and cortical bone as well as other trabecular parameters obtained from µCT scanning of femurs from aged females (≥ 52 weeks of age) of distal and midshaft regions (N = 3, 3). e, Representative images of µCT-scans. Unpaired Student T-Test 2-tailed for panels a-c. *p < 0.05, **p < 0.01, ns = not significant. Error Bars ± SEM.
