Extended Data Fig. 1: Sex-Dependent High Bone Mass in Genetic Models that Target KNDy ARC Neurons Occurs By 4 Weeks of Age.
a, Representative µCT images of female and male distal femurs at 4.5 and 6.5 weeks. b, Box and whisker plots of structural bone parameters of control Esr1fl/fl and mutant Esr1Pdyn-Cre (mutant) females (red) and males (blue); legend on top. c, Time course of high bone mass in Esr1fl/fl (black) or Esr1Nkx2.1-Cre (red) females beginning at 1 week of age, data for the 4.5-week time point are taken from2 and re-graphed, (N = 4 for all groups except N = 2 for mutant at 4 wks), legend on top. d, µCT imaging of females at 3 and 4 weeks of age. e, Modified Pentachrome staining of sections from control and mutant distal femur 4 weeks of age showing enhanced mineralized bone (red) in the mutant femur. One-Way ANOVA for panel b (Šidák’s multiple-comparisons test). Two-Way ANOVA for panel c, F(1, 21) = 65.63, P < 0.0001. ****p < 0.0001, ns = not significant. Error Bars ± SEM.
