Extended Data Fig. 2: Expression of TMEFF1 in tissues and CNS cell types.
From: TMEFF1 is a neuron-specific restriction factor for herpes simplex virus
a, Domain structure of the TMEFF1 protein. A transmembrane protein with an EGF-like and two Follistatin-like domains in the extracellular region, a transmembrane region, and a cytoplasmic domain of unknown structure. SP signal peptide; FSLD: follistatin-like domain; EGF, EGF-like domain; TM, transmembrane domain; CTD, cytoplasmic domain. b, Expression of TMEFF1 mRNA in different human tissues. TPM, transcripts per million. The graph is based on publically available data from Proteinatlas.org (date of data retrieval: August 1, 2022). c, Expression of TMEFF1 mRNA relative to BACTIN in different cells representing cell types found in the human brain. Total RNA from iPSC-derived cortical neurons, astrocytes, microglia, and oligodendrocyte progenitor cells was analyzed for expression of TMEFF1 mRNA by RT-qPCR (n = 2). d, UMI data on TMEFF1 transcripts in annotated cell types in the cerebral cortex from previously published single-nucleus RNA sequencing data (ref. 50). VSMCs, Vascular smooth muscle cells. e, Total RNA was isolated from different regions of C57BL/6 mouse brain (n = 1), analyzed for Tmeff1 mRNA levels by RT-qPCR. Data were normalized against Bactin. f, HEK293T cells were transfected with increasing amounts of mouse Tmeff1 expression plasmid and infected with HSV-1-GFP. Percentage of GFP-positive cells was evaluated 12 h p.i (n = 3). Data are shown as means of biological sample replicates ± s.d. Statistical analysis was performed using a two-tailed one-way ANOVA test followed by unpaired t-tests of means indicated by P-values (c + e). P < 0.05 was considered statistically significant.
