Extended Data Fig. 4: Generation and characterization of Tmeff1^−/− mice.

a, Illustration of the targeting region of the gene trapping cassette used to generate Tmeff1^−/− mice. The arrows in red represent the primers used for analysis og mouse genotypes. b, PCR analysis of DNA from Tmeff1^+/+ (WT), Tmeff1^+/− (HET), and Tmeff1^−/− (HOM) mice (littermates) using the primers pairs 1 and 2 versus 1 and 3 (Gel image representative of the standard genotyping of all HET x HET litters). c, Genomic sequences from WT and HOM mice around the genomic region targeted by the gene trapping cassette. d, Total RNA was isolated from the brains of C57BL/6N and HOM mice, analyzed for Tmeff1 mRNA levels by RT-qPCR. Data were normalized against Bactin levels (n = 3 mice /genotype). Tmeff1 expression in WT and HOM mice were compared using an unpaired two-tailed t-test. P < 0.05 was considered significant. e, Brain lysates from WT and HOM mice were immunoblotted for TMEFF1 (representative of 2 individual repeats). f-g, WT, Irf3^−/−, Ifnar1^−/−, and Tmeff1^−/− mice were infected in the cornea with 2 × 10⁶ PFU/eye HSV-1 (Mckrae) and monitored for f, Weight change (day 5 p.i.), and g, Disease symptoms (day 5 p.i.). Each dot represents one animal (Mock infected, WT n = 5; Infected WT n = 12, Tmeff1^−/− n = 7, Irf3^−/− n = 5, Ifnar1^−/− n = 4). Two-tailed one-way ANOVA test for difference of means ± s.d., followed by unpaired two-tailed t-tests of means of groups indicated by P-values. P-values < 0.05 were considered statistically significant. For gel source data, see Supplementary Fig. 1.

Source Data