Fig. 3: Targeted adenine base editing of E. coli MG1655-bla in the gut of BALB/c mice.
From: In situ targeted base editing of bacteria in the mouse gut
a, Summary of the experimental set-up. Five days following colonization, mice were treated with packaged cosmids equipped with gpJ 1A2 and the λ-P2 STF chimera, and an ABE targeting the bla gene. In one arm we investigated dose–response and in the other the impact of multiple doses on treatment efficacy, following animals up to 6 weeks (w). Dose titres were measured as transducing units (tu). b, Editing efficiency at different time points for a single dose with decreasing concentrations. Points show individual mice (n = 10 per group), bars indicate median ± 95% confidence interval (****P < 0.0001 by one-way analysis of variance (ANOVA) with Tukey’s multiple-comparisons test). c, Editing efficiency following multiple treatments (P values indicated on the graph; not significant (NS), P > 0.05 by repeated-measures ANOVA with Tukey’s multiple-comparisons test; treatments are indicated by black arrowheads on the x axis; n = 9 animals). Bars represent mean ± s.d. d, Number of copies of payload (cp) recovered in the stool and quantified by ddPCR. Bars represent the group median (P values indicated on the graph; NS P > 0.05 by Kruskal–Wallis test with Dunn’s multiple-comparisons test; n = 10 animals). D0, day 0; T0, time 0. Panel a created with BioRender.com.
