Extended Data Fig. 2: Cell receptor analysis for efficient cosmid delivery.
From: In situ targeted base editing of bacteria in the mouse gut
Delivery efficiency assays of cosmids harboring Ur-λ wild-type (WT) gpJ and stf, or harboring gpJ chimeras (1A2 or A8) and P2 STF. Transduction of a payload encoding a sfGFP fluorescent protein gene was measured in a flow cytometer (excitation: 488 nm, emission: 530/30 BP; Attune NxT Thermo Scientific) at different MOIs. a) Delivery efficiency into MG1655 WT, carrying the endogenous OmpC variant in the genome. b) and c) Delivery efficiency into a MG1655 strain deleted for both lamB and ompC genes, complemented with two different ompC variants on a plasmid (p1471 or p1472). d) Delivery efficiencies in E. coli MG1655 of λ particles carrying the WT λ STF (purple line), no STF (blue line) and λ-P2 STF chimera (p938) (orange line).
