Extended Data Fig. 4: Multiplicity of injection (MOI)-dependent delivery efficiency and corresponding adenine base editing (ABE) and cytosine base editing (CBE).
From: In situ targeted base editing of bacteria in the mouse gut
Delivery efficiency was obtained by colony counting after overnight incubation on LB and LB (chloramphenicol) agar plates. Left y axis: The percentage delivery was obtained by dividing the number of colonies on chloramphenicol plates by the number on LB plates (blue line). Right y axis: Base editing of β-lactamase in strain MG1655-bla diminishes cell growth on carbenicillin plates (colony-forming units cfu per µl; orange line). For the control samples, the gRNA targeting bla was replaced by a non-targeting SapI spacer. The graphs show the average and standard deviation of an experiment performed in triplicate.
