Extended Data Fig. 6: Crystal structure of mature ProteinTS224A inactive variant.
From: An engineered enzyme embedded into PLA to make self-biodegradable plastic
a, The monomeric structure is presented as a cartoon representation. The three amino acid residues (D39, H72, S224A) forming the catalytic triad are shown as magenta-colored sticks. Three-dimensional structure of ProteinT with the prodomain and catalytic domain colored in orange and cyan, respectively. Conserved DS1 and DS2 disulfide bonds are labeled and shown as yellow sticks. Calcium ion is colored in pink and magnesium ion in green. b, representation of calcium ion coordination. c, representation of magnesium ion coordination.
