Extended Data Fig. 7: Biodegradation assessment of enzymated PLA material.
From: An engineered enzyme embedded into PLA to make self-biodegradable plastic
a, Aqueous biodegradation of enzymated PLA material after a long-term storage of 18 months at room temperature. Depolymerization of PLA + MBs + ProteinTFLTIER film (line) or PLA + MBs + Proteinase K film (dotted line) at pH 9.0 and 45 °C before (light blue) and after (black) a long-term storage of 18 months at room temperature of the films. Each filled symbol represents the mean value ± s.d. (n = 2 independent experiments). b, Aqueous biodegradation of enzymated PLA material after 1 month immersion in yogurt. Depolymerization of PLA + MBs + ProteinTFLTIER film at pH 9.0 and 45 °C, at the time of the film production (blue), after a long-term storage of 18 months at room temperature (black), and after a long-term storage of 18 months at room temperature followed by an additional 1 month immersion in yogurt at 4 °C (red). Each filled symbol represents the mean value ± s.d. (n = 2 independent experiments), except for yogurt at 4 °C (n = 1).
