Fig. 5: Three-dimensional variability analysis reveals Pil1/Lsp1 lattice stretching and its effects on membrane organization within native-source MCC–eisosomes.
From: Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain
a, Well-defined two-part density (green for PI(4,5)P2 and dodger blue for putative PS) occupying the charged pocket in a deepEMhancer sharpened map of the most compact protein lattice conformation (model in dark red). b, No clear density is observed in the charged pocket in a sharpened map of the most stretched protein lattice conformation (model in light yellow). c,d, Membrane void pattern, or lack thereof, in corresponding slices of unsharpened maps of the most compact protein lattice (c) and the most stretched protein lattice (d) conformation. e, Model illustrating that lattice stretching destabilizes lipid headgroup and sterol binding (compact model in dark red, stretched model in light yellow, PI(4,5)P2 headgroup in green, PS headgroup in dodger blue and sterols in violet).
