Extended Data Fig. 5: B cell expansion in the SED of the NALT requires interactions with T cells.
From: Turbinate-homing IgA-secreting cells originate in the nasal lymphoid tissues
(A, B) Representative flow cytometry plots and quantification of TFH cells in the MedLN derived from WT host mice that were adoptively transferred with H2-Ab+/+ or H2-Ab−/− Rosa26tdTomato/+ B1-8hi B cells and CD45.1+ CD4+ OT-II T cells 5 days following immunization with i.n. NP-OVA + MPLA. PD1+ CXCR5+ population gated from CD62L− CD4+ CD45.1+ OT-II T cell compartment. *p = 0.05; p = 0.60. n = 6; 2 independent experiments; unpaired two-tailed Student’s t-test; data represent mean±s.e.m. (C, D) Representative plots and quantification of GC cells in NALT (C) and MedLN (D) at day 7 following i.n. NP-OVA + MPLA with or without injection of CD4+ OT-II T cells. FAS+ CD38- GC population gated from the B220+ compartment. C, p = 0.064; D, p = 0.15. n = 6; 2 independent experiments; unpaired two-tailed Student’s t-test; data represent mean ± s.e.m. (E) Host mice were injected with anti-CD4 mAbs while transferring GFP+ B1-8hi B cells (as in Fig. 2g). Flow cytometry analysis of CD4+ T cells in the NALT at day 7 following NP-OVA i.n. boost. CD4+ T cell population gated from the CD45+ compartment. ****p = 1 × 10−5. (F-H) Experimental setting as in E. Flow cytometry quantification of B cells in NALT at day 7 following NP-OVA i.n. boost. In F, FAS+ CD38- GC population gated from B220+ compartment, p = 0.135; in G, GFP+ B1-8hi population gated from FAS+ CD38- GC compartment, *p = 0.0156; in H, IgA+ GFP+ B1-8hi B cell population gated from the B220+ compartment, **p = 0.0015; control n = 6, anti-CD4 n = 7; 2 independent experiments; unpaired two-tailed Student’s t-test; data represent mean ± s.e.m.
