Extended Data Fig. 6: Antigen-specific B cells are primarily detected in the turbinates region of i.n. vaccinated mice.
From: Turbinate-homing IgA-secreting cells originate in the nasal lymphoid tissues
(A) TPLSM Z-stack images of GFP+ B1-8hi B cells in different tissues at day 7 post i.n. NP-OVA + MPLA boost. WT host mice were adoptively transferred with GFP+ B1-8hi B cells and Rosa26tdTomato/+ CD4+ OT-II T cells following i.n. prime and boost immunizations followed by TPLSM imaging. Scale bars, 70–200 µm. (B) TPLSM Z-stack images of the LNs after different vaccination strategies. i.n.: NP-OVA + MPLA prime and boost; i.p.+s.c.: NP-OVA+Alum i.p. prime and NP-OVA + MPLA s.c. boost; oral gavage with NP-CT. 2 independent experiment. See Supplementary Data Table 2. (C) Confocal images of the NT and TPLSM images of the NALTs after vaccination with NP-OVA in different nasal adjuvants. Numbers indicate the fraction of mice with NT B cells. 2 independent experiment. (D) TPLSM image of B1-8hi B cells in the NT. WT host mice were adoptively transferred with Blimp-1-YFP Rosa26tdTomato/+ B1-8hi B cells and CD4+ OT-II T cells following i.n. NP-OVA + MPLA prime and boost vaccination. Upper mouse heads were collected 7 days following boost. Scale bars, 50 µm. n = 5; 2 independent experiments.
