Extended Data Fig. 4: Transferred antigen-specific T cells express activation markers in the NALT in response to vaccination.
From: Turbinate-homing IgA-secreting cells originate in the nasal lymphoid tissues
(A, B) Representative TPLSM images of the NALT at day 3 post i.n. NP-OVA + MPLA. NALTs were derived from WT host mice adoptively transferred with GFP+ B1-8hi B cells and Rosa26tdTomato/+ CD4+ OT-II T cells following i.n. NP-OVA + MPLA (A), or transferred with 107 naive CFP+ B cells 1 day after i.n. NP-OVA + MPLA (B). Merged Z-stack. Scale bars, 30–150 µm. n = 3 in each experiment. (C, D) Activation marker expression on transferred OT-II T cells at day 1.5 and day 3 following i.n. NP-OVA + MPLA. NALT cells were derived from WT host mice adoptively transferred with GFP+ B1-8hi B cells and Rosa26tdTomato/+ CD4+ OT-II T cells following i.n. NP-OVA + MPLA. CD69+ and CD44high CD62L− population gated from the Rosa26tdTomato/+ CD4+ T cell compartment. C: **p = 0.0022, *p = 0.0436, ***p = 0.0002; D: **p = 0.0013, p = 0.0887, ***p = 0.0001; unimm n = 5, day 1.5, n = 7, day 3 n = 8; 2 independent experiments; unpaired two-tailed Student’s t-test; data represent mean ± s.e.m.
