Fig. 2: Perisomatic region of cortical cells.

a, A measure of the distance from the soma for each edit that was made to the segmentation during proofreading of 1,347 cells. The teal line denotes the average and the shading marks the 10th–90th percentile across all cells. The arrow marks 15 µm. b, Example cell demonstrating the extent of mesh information used to extract somatic, nuclear and synapse features. All cell meshes were restricted to 15 µm from the centre of the nucleus. c, Representative example of nuclear infolding in a single electron microscopy image. The soma is highlighted in grey, black represents the nuclear envelope and orange marks the areas classified as infolded on the basis of the shrink-wrap method (Methods). d, Example cell demonstrating the extent of mesh information used to extract postsynaptic features (left) and three example PSSs (right). All synapses included in the PSS analyses were within 60 µm from the centre of the nucleus. e, A 3D rendering of the somatic cutouts from all the cells from a 100-µm column that was densely reconstructed for which manual labels were given (n = 1,619). Cells rendered are organized by their cell class and coloured by their cell subclass according to the colour scheme displayed. Scale bars, 5 µm (b), 2 µm (c) and 20 µm (d).