Extended Data Fig. 2: Tissue tropism in mice intranasally inoculated with bovine H5N1 virus, a replicate experiment.
From: Pathogenicity and transmissibility of bovine H5N1 influenza virus
BALB/cJ mice (7 weeks old, n = 10 biologically independent animals per virus) were deeply anaesthetized and intranasally inoculated with 103 PFU of A/dairy cattle/New Mexico/A240920343-93/2024 (H5N1; ‘Cow-H5N1’) or A/Vietnam/1203/2004 (H5N1; ‘VN1203-H5N1’) in 50 μl of PBS. At 3 and 6 days post-infection, five mice in each group were euthanised and tissues were collected for plaque assays in MDCK cells. In the figure panels, the floating bars show the median titre for each tissue of each inoculation group and variability is represented by the range. When virus was not detected in a tissue, an arbitrary value below the limit of detection was assigned to enable visualization of the datapoint on the graph. PFU/g, plaque-forming units per gram of tissue.
