Extended Data Fig. 4: Somatic mutational landscape of mitochondrial genomes in colorectal cancer.

a, Oncoplot of somatic mitochondrial DNA gene (rows) mutations in 1,027 (97%) of the 1,063 sequenced tumours (columns). The TMB for each sample is presented at the top and the number of tumours with the mutation is shown on the right, coloured by mutation type. b, Variant allele frequency (VAF) accumulation curves for missense, silent and truncating mitochondria mutations (one-tailed F-test). c, dN/dS ratio for mtDNA somatic missense mutations by different VAF cut-offs. The numbers of missense and silent mutations for different VAF cut-offs were indicated. The error bars represent the 95% confidence intervals of the dN/dS ratio (likelihood). d, Total amount of mitochondrial mutations displayed per age group with one-way ANOVA comparison. The boxes represent the interquartile ranges (IQRs) between the first and third quartiles, the centre line represents the median, and the whiskers extend 1.5 times the IQR from the top and bottom of the box. The numbers of tumours in each age group are shown at the bottom of the box plots and mean values are shown as black dots. e, Mutually exclusive or co-occurring mitochondrial gene mutations in all tumours with Fisher’s Exact test adjusted by Benjamini-Hochberg False Discovery Rate (* FDR < 0.05 and ▪ FDR < 0.1). The number of patients with the mutation is shown inside brackets next to the gene name.