Extended Data Fig. 6: AriA and AriB interact to form compositional and conformationally heterogeneous complexes.
From: Architecture and activation mechanism of the bacterial PARIS defence system
a, The final nine cryo-EM reconstructions for the AriAEQ-AriBE90A complex, colour-coded by local resolution from <3 Å (blue) to >7 Å (red). The final resolution and the number of particles corresponding to each complex form are noted at the bottom of each map (see Supplementary Fig. 4 for reconstruction and data processing). b, c, cryo-EM density map and cartoon structural representations of the AriA6-AriB2 and AriA6-AriB3 complexes. d, A cartoon model depicting steric clashes between Up and Down AriB monomers at the ATPase head dimer. Only one AriB can interact with a given AriA HH interface. e, Left: Cryo-EM structural representations of the AriA6-AriB3 complex showing the relative orientations (Up/Down) of AriB. Right: A simplified schematic illustrating AriA-AriB complex formation and AriB Up/Down designations.
