Extended Data Fig. 2: Cryo-EM workflow for AriAEQ.
From: Architecture and activation mechanism of the bacterial PARIS defence system
a, Representative micrograph (from 5,174 micrograph dataset) displaying the AriAEQ sample. Scale bar = 100 nm. b, Workflow outlining the cryo-EM reconstruction process for AriAEQ using cryoSPARC. c, Representative 2D classes displaying AriA samples from the final particle stack, with the highlighted yellow classes also depicted in Fig. 2b. Scale bar = 10 nm. d, Gold-standard FSC curve for the final global refinement of AriAEQ. e, Two views of the globally refined cryo-EM map for the AriAEQ hexamer, colour-coded by local resolution from <3 Å (blue) to >7 Å (red). f, Gold-standard FSC curve for the masked local refinement of AriAEQ. g, A view depicting the locally refined cryo-EM map for the AriAEQ hexamer, colour-coded by local resolution from <3 Å (blue) to >7 Å (red). h, Example cryo-EM density with a built atomic model demonstrating the model fit. Yellow and blue are two AriAEQ protomers.
