Extended Data Fig. 4: Transport activity and surface expression of DAT^WT and its mutants.

a, Quantitation of the transport activity in DAT^WT and its mutants using the [³H]DA uptake assay. The transport activity of DAT^WT and its mutants is normalized to their respective expression level and the transport activity of DAT^WT was set to 100%. The uptake duration is set to 2 min to ensure that the uptake occurs within the linear range. Data represent the mean ± S.E.M. n = 3 biologically independent experiments. b, Representative western blots of the surface DAT^WT and its mutants. Biotinylated Na⁺/K⁺ ATPase on the cell surface was used as the loading control. Experiment was repeated three times independently with similar results. For gel source data, see Supplementary Fig. 1. c, Densitometric quantification of surface expression for DAT^WT and its mutants. The gels derived from the same experiment were processed in parallel. The surface expression of DAT^WT and its mutants was first normalized to Na⁺/K⁺ ATPase and then to the expression of DAT^WT. Each circular symbol represents an individual data point. Data are represented as mean ± S.E.M. n = 3 biologically independent experiments.

Source Data