Extended Data Fig. 4: Size-exclusion chromatography analysis of peptide-induced switching.
From: De novo design of allosterically switchable protein assemblies
For all designs 5 μM protein was run without peptide (pink) and with 10 μM cognate peptide (blue). Traces represent normalized UV absorbance at 280 nm. Peak near ~20 corresponds to unbound free peptide. Green colored boxes indicate designs with appreciable change in elution profile when peptide is added, in the expected direction. Names of designs isolated for further testing are highlighted in wheat.
