Extended Data Fig. 6: Fluorescence Polarization quantification of affinity of rings.
From: De novo design of allosterically switchable protein assemblies
Protein is titrated down in 2-fold dilutions from 10 μM across 24 wells at a constant concentration of 1 nM TAMRA-labelled peptide. Estimates of dissociation constant (Kd) and standard error from four replicate curves are printed in the bottom right.
