Extended Data Fig. 5: Neutrophil NET formation drives arthritis disease severity in MICL−/− mice.
From: Recognition and control of neutrophil extracellular trap formation by MICL
a, Representative Sytox green fluorescent and bright field images of MSU-induced NETs in thioglycollate-elicited neutrophils in the presence of 100 μM BB-Cl-amidine or 10 μM GSK484. Scale bar = 200 µm. b, Schematic representation of BB-Cl-amidine treatment regime during CAIA and severity scoring of WT and KO mice during CAIA treated with vehicle or PAD4 inhibitor. Black arrow indicates start of the treatment. Data are represented as mean ± SEM (pooled data from two independent experiments with 5 mice/group/experiment). c, Schematic representation of GSK484 treatment regime during K/BxN serum transfer model and severity scoring of WT and KO mice during K/BxN serum transfer model treated with vehicle or GSK484. Black arrow indicates start of the treatment. Data is a representative example of n = 2 independent experiments, mean ± SD, 5 mice/group/experiment. d, Schematic representation of DNaseI treatment regime and severity scoring of WT and KO mice during CAIA (n = 1 experiment with 6 mice/group). Black arrow indicates start of the treatment. b,c,d, Statistical significance was determined by two-way ANOVA with Tukey’s post hoc test. WT, wild-type mice; KO, MICL−/− mice; ns, not significant; *, p < 0.05. Schematics in panels b–d were created using BioRender (https://biorender.com).
