Extended Data Fig. 2: MICL is required for controling neutrophil responses during arthritis.
From: Recognition and control of neutrophil extracellular trap formation by MICL
a, Schematic of K/BxN serum transfer model and severity scoring shown as mean ± SD analysed over time. b, Total live cell populations (defined by gating strategy in Extended Data Fig. 1b) isolated from inflamed joints on day 10, mean ± SD. Neutrophils WT vs MICL−/− p = 0.0086. a,b, Data is a representative example of three independent experiments, with 4 mice/group/experiment, and analysed using two-way ANOVA with Bonferroni’s multiple comparisons test. Schematic in panel a was created using BioRender (https://biorender.com). c,d, tSNE plots of CD45+ myeloid populations displayed as neutrophils (blue), Ly6Chigh cells (green), Ly6Clow cells (orange), antigen presenting cells (APCs) (pale violet), and all other subpopulation (grey) in the bone marrow (c), and blood (d) of naïve mice. Data is a representative example of three independent experiments, with 4 mice/group/experiment, represented as mean ± SD. e,f, Fold change in mean fluorescent intensity (MFI), relative to wild-type mice of (e) neutrophil activation markers (CD11b, CD18, CD62L) and (f) chemokine receptors (CXCR2, CCR1, C5aR), isolated concomitantly from joints or blood of arthritic mice during CAIA on day 7 (pooled data from two independent, n = 10 biologically independent mice). g, Fold change in mean fluorescence intensity (MFI) on neutrophils, relative to wild-type mice of CD11b, CD18, CD62L and CCR1, isolated from the joints of arthritic mice on day 10. Data is a representative example of n = 3 independent experiments with 4 mice/group/experiment, shown as mean ± SD and analysed using two-way ANOVA with Bonferroni’s multiple comparisons test. h,j, Fold change in mean fluorescent intensity (MFI) relative to wild-type mice of neutrophil activation markers (CD11b, CD18, CD62L) and chemokine receptors (CXCR2, CCR1), isolated concomitantly from bone marrow (h) and blood (j) of naïve mice (representative example of n = 2 experiments with 5 mice/group/experiment). WT, wild-type mice; KO, MICL−/− mice; *p < 0.05; ns, not significant.
