Extended Data Fig. 3: Neutrophil depletion reduces inflammation in WT and MICL−/− mice during arthritis.
From: Recognition and control of neutrophil extracellular trap formation by MICL
a, Flow cytometry gating strategy used to confirm αLy6G antibody-mediated neutrophil depletion in CAIA model at day 9. Neutrophils were identified as CD45+CD11b+SSChigh cells. b, Schematic representation of the αGR-1-mediated neutrophil depletion strategy in the CAIA model. Schematic in panel b was created using BioRender (https://biorender.com). c, Flow cytometry contour plot-illustrating depletion of peripheral blood neutrophils 48 h post-injection with αGR-1. d, Severity scoring of WT and KO mice treated with isotype or αGR-1 antibodies, as indicated (pooled data from two independent experiments, n = 12 biologically independent mice). Data is represented as mean ± SEM. e, Quantification of neutrophils (CD45+CD11b+SSChi) and monocytes (CD45+CD11b+F4/80+) in the peripheral blood on day 11. n = 4 (neutrophils) or n = 3 (monocytes) biologically independent mice represented as mean ± SD. f, Contour plot showing purity of neutrophils after bone marrow isolation. g, Pseudo-colour plot illustrating endogenous and cell-tracker labelled, adoptively transferred CD45+CD11b+Ly6G+ neutrophils in the joint at day 9 (48 h post-transfer).WT, wild-type mice; KO, MICL−/− mice; Ab, αGR-1 or isotype; ns, not significant; *, p < 0.05.
