Extended Data Fig. 6: Analysis pertaining to Walk-OFF and Brake mechanisms.
From: Neural circuit mechanisms underlying context-specific halting in Drosophila
a. DNs recruited by BPN and P9 (magenta: contralateral descending; blue: ipsilateral descending; grey: unidentified descending arbors). b,c. Scatter plot showing DNs downstream of (b) BPN and (c) P9 differentially affected by FG versus BB. d. Expression pattern of split-Gal4 lines covering oDN1 and BDN2. (CsChrimson-mVenus: green, neuropil: magenta, scale bars: 50 μm). e. Trial averaged translational velocity (left) and angular velocity (right) of decapitated, tethered flies while activating P9, oDN1, or BDN2. 3 s light stimulation (red bars) starts at vertical stippled lines in velocity plots. n = 7-10 flies per genotype, mean ± s.e.m. f. Probability density of front leg joint angle (body-coxa, coxa-trochanter and tibia-tarsus) if BRK or FG was optogenetically activated during swing phase (top) or during stance phase (bottom). n = 7-33 trials averaged across 6-17 flies on the ball. g. Light microscopy images of combined split-Gal4 lines for co-labelling BRK and BDN2. Magenta arrowheads in the brain indicate BDN’s soma (left), empty arrowhead in the VNC indicate BRK’s soma (right). h. EM segmentation of BRK and BDN2 in the brain (left) and VNC (right). Supplementary Table 3 describes all connectome IDs. Supplementary Table 1 shows full genotypes and exact sample sizes.
