Extended Data Fig. 8: Additional quantification of map comparisons.

a, Summary of the changes in telencephalic PCs by directly comparing the maps across two sessions in the microscope reference frame. Experiments from Fig. 4 and Fig. 5 are represented on the horizontal axis: chamber rotation (Fig. 4m), landmark removal (Fig. 4e), wall morphing (Fig. 4i), wall rotation (Fig. 5m), fish removal with no change in the chamber (Fig. 5a), landmark removal with fish removal (Fig. 5e), wall morphing with fish removal (Fig. 5i), and wall morphing with landmark removal and fish removal (Fig. 6a). From top to bottom are place field correlation (PF correlation), population vector correlation (PV correlation), and the shift in the place field location (PF shift, Methods). The union of telencephalic PCs from both sessions was used. The comparison between the 1^st and the 2^nd sessions (red) is plotted against a reference comparison between the early (1^st half) and late (2^nd half) stages of the 1^st session (black). Solid lines are the mean distributions across all fish, and the shaded region indicates the standard deviation across all fish. Vertical dashed lines are the medians of the averaged distributions. b, similar to a but after registering the maps from the 2^nd session to the 1^st session with reference to the chamber wall geometry and landmarks (Methods). c, similar to b but the transformation is done by finding the rotation of the reference anchor points that maximizes the mean PF correlation after registration (Methods). d, The mean PF correlation as a function of the anchor rotation angle used in the nonrigid transformation, for different experiments (Methods). e, Summary of changes in the spatial activity maps of telencephalic PCs under different environmental manipulations after applying the nonrigid transformation with the best rotation angle. The median of PF correlation, PV correlation, and PF shift are plotted for each fish. Red dots are comparisons made between the 1^st session and the 2^nd session. Gray dots are reference comparisons made between the early (1^st half) and late (2^nd half) stages of the 1^st session (also after nonrigid transformation with the best rotation angle). The results of statistical tests are marked for each fish separately (one-sided Wilcoxon signed-rank test, *** for p < 10⁻⁵, ** for p < 0.001, * for p < 0.01, n.s. for p ≥ 0.01). See Supplementary Table 1 for exact p-values. f, Analysis of fish removal experiment as in Fig. 5d, but only for individual fish where the olfactory cues were cleaned thoroughly before each recording session with soap and isopropanol. The results of statistical tests for different measures against a control comparison between the early (1^st half) and late (2^nd half) period of the 1^st session are shown for each fish (one-sided Wilcoxon signed-rank test, same for Extended Data Fig. 8g, fish 1 and fish 2 corresponds to fish 1 and fish 2 in Supplementary Table 1). g, Analysis of fish removal experiment as in Fig. 5d, but only for individual fish where the bottom was rotated without cleaning to scramble any potential olfactory cues (fish 1 and fish 2 correspond to fish 3 and fish 4 in Supplementary Table 1). See Supplementary Table 1 for exact p-values.