Fig. 3: Activity manifold of PCs untangles across time.
From: A population code for spatial representation in the zebrafish telencephalon
a, Change in manifold structure across two stages of the experiment (early, 0–30 min; late, 60–90 min). Individual time points are colour coded by the animal’s location within the behavioural arena. b, Mean physical distance to 30 neighbouring points in the two-dimensional manifold space, averaged across all time points (left). Change in this distance between early and late stages of the experiment (blue) and baseline (black, 30 randomly selected timepoints rather than neighbours; Methods). Each animal is shown individually. Changes within animals were tested using one-sided Mann–Whitney U-test. c, Change in mean spatial specificity across telencephalic PCs from early to late stages of the experiment. Mean spatial specificity of PCs is baseline corrected by subtracting the mean spatial specificity of an equal number of random cells. Each animal is shown individually. One-sided Mann–Whitney U-test is used to compare changes in PCs and those in random cells for individual animals. d, Change in mean decoder error from early to late stages of the experiment. Each animal is shown individually, and changes in decoder error within animals are tested using a one-sided Mann–Whitney U-test. e, Change in PF size from early to late stages of the experiment. Each animal is shown individually. Changes within animals are tested using one-sided Wilcoxon signed-rank test. f, Example spatial activity maps for early and late stages of the experiment. Each row is represents one PC. For the whole figure, telencephalic PCs used are defined across the experiment, excluding the first and last 15 min, and behavioural coverage was equalized between the two examined time intervals before making comparisons (Methods). Statistical significance ***P < 10−5, **P < 0.001, *P < 0.01, not significant (NS) P ≥ 0.01. See Supplementary Table 1 for exact P values of all statistical tests. Scale bar, 10 mm.
