Extended Data Fig. 6: Expression and production of IL-3 by GD3 cells.
From: A γδ T cell–IL-3 axis controls allergic responses through sensory neurons
a, QPCR of Il3 (normalized to Gapdh) from FACS sorted γδ T cells from naïve WT pinnae (n = 3 per group, except GD3 cells (n = 4)). b, Flow cytometric quantification of naive Il3GFPfl/fl pinnae (n = 8 per group). c,d, Flow cytometric quantification of MACS enriched GD3 cells from Skint1-/- FVB/N pinnae (c) or FACS sorted γδ T cells from Il3GFPfl/fl pinnae (d) left unstimulated (unstim) or stimulated as indicated (c, n = 3 per group; d, n = 2 per group). Symbols represent individual experimental replicates of pooled mice (a,c,d) or mice (b). Bar plots are mean ± SEM. Data represent at least two independent experiments and were combined. n = 3-4 experimental replicates of pooled mice (a), n = 8 mice (b), or n = 2-3 experimental replicates (c,d). Statistical tests: one-way ANOVA with Tukey’s multiple comparisons test (a-c). * p < 0.05, ** p < 0.01, ns=not significant.
