Extended Data Fig. 7: IL-3 targets PEP1 sensory neurons, which encode mixed itch and pain and are required for allergic itch.
From: A γδ T cell–IL-3 axis controls allergic responses through sensory neurons
a-c, Cumulative cheek scratch bouts following i.d. injection of recombinant cytokine, then allergen or IL-31 (a, n = 8 HDM sham; n = 7 HDM IL-3; n = 12 Alternaria sham; n = 11 Alternaria IL-3; n = 8 fire ant; b, n = 11 sham; n = 9 IL-3; c, n = 12 papain sham; n = 8 papain IL-17A; n = 15 papain sham; n = 12 papain CCL3). d, Cumulative cheek scratch bouts following i.d. injection of papain (n = 19 WT; n = 20 Il4ra-/-). e, Representative traces of ratiometric calcium imaging of DRG neurons sequentially stimulated as indicated. f, Venn diagram indicating the overlapping responsiveness of individual DRG neurons. g, Percent of DRG neurons that responded to the indicated stimuli (n = 14 per group). h, Percent of IL-3 responsive DRG neurons that also responded to papain, capsaicin, or AITC (n = 14 per group). i, Flow cytometric quantification of naive pinnae (n = 10 WT; n = 8 Trpv1DTR). j, Cumulative cheek wipe or scratch bouts following i.d. injection of CNO (n = 10 Tac1cre/+; n = 8 Tac1cre/+GqDREADD). k, Cumulative cheek wipe or scratch bouts following i.d. injection of CNO, then papain or IL-31 (n = 9 GiDREADD papain; n = 11 Tac1cre/+GiDREADD papain; n = 7 GiDREADD IL-31; n = 8 Tac1cre/+GiDREADD IL-31). Symbols represent mice (a-d,i,k) or individual wells (g-h). Violin plots show the median and quartiles. Bar plots and time courses are mean ± SEM. Data represent at least two independent experiments and were combined. Statistical tests: two-sided unpaired t-test (a, HDM and fire ant; b,c; i, dermal γδ T cells and DETCs; k, IL-31), two-sided Mann Whitney U-test (a, Alternaria; d; i, GD3 cells; k, papain), one-way ANOVA with Tukey’s multiple comparisons test (h), or two-way ANOVA with Tukey’s multiple comparisons test (j). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns=not significant.
