Extended Data Fig. 5: Vγ4+ GD3 cells are the major source of IL-3 in naive skin.
From: A γδ T cell–IL-3 axis controls allergic responses through sensory neurons
a, Heatmap of multiplex assay of cell-free supernatant from FACS sorted γδ T cells stimulated as indicated (n = 3 per group). * symbols represent a cytokine or chemokine that significantly increased following stimulation. b,c,e, IL-17A or IL-3 ELISA of cell-free supernatant from FACS sorted γδ T cells or immune cells stimulated as indicated (b, n = 4 per group, except stimulated GD3 cells (n = 6); c, n = 3 per group; e, n = 2 per group). d, Flow cytometry of FACS isolated γδ T cells. CTV = cell trace violet (viability: n = 3 per group; CTV: n = 2 per group). Symbols represent individual wells (b) or experimental replicates (c-e). Bar plots are mean ± SEM. Data represent at least two independent experiments combined, except in a, a single pilot experiment, and b, a representative experiment with n = 4–6 wells. Statistical tests: two-way ANOVA with Tukey’s multiple comparisons test (a-c) or two-way ANOVA (d, viability). * p < 0.05, ** p < 0.01, **** p < 0.0001, ns=not significant.
