Fig. 2: Visually driven 5-HT signalling inhibits P1 and plP10 courtship-promoting neurons.
a, P1 Sp-GAL4-driving UAS-mCD8-GFP (green) in the adult brain. Anti-nC82 is in magenta. Scale bar, 50 μm. b, P1 > CsChrimson males continue courtship upon artificial activation despite the threat (n = 30 each (no threat); n = 30, 28 and 30 (threat)). c, Predicted path connecting LC16 to pC1a through 5-HTPMPD01 (top), and electron microscope reconstructions of female pC1 and 5-HTPMPD01 neurons (bottom). See Supplementary Information Fig. 2c for axis details. d, TRHR23E12 Sp-GAL4 labels PMPD neurons (top), and 5-HT clusters in the adult brain (bottom). 1 denotes the posterior medial dorsal protocerebrum (PMPD) cluster. See Supplementary Information Fig. 2d for the nomenclature for clusters. e, ∆F/F0 (%) of the 5-HTPMPD > GCaMP6s signal after artificial activation of LC16 neurons (left), and the mean ∆F/F0 (%) at baseline versus first stimulation (n = 7). f, TRHR23E12 > TNT males fail to stop courting in response to the threat (n = 15, 15 and 17 (no threat); n = 14, 19 and 24 (threat)). g, TRHR23E12 > CsChrimson (CsChr.) males stop courtship upon artificial activation without threat (n = 17, 19 and 25 (red light OFF); n = 14, 22 and 19 (red light ON)). h, P1 > 5-HT7 RNAi flies respond less to the threat (n = 22, 16 and 24 (no threat); n = 39, 24 and 39 (threat)). i,j, ∆F/F0 (%) of the P1 > GCaMP6s signal pre-application and post-application of 100 µM 5-HT without (i) or with (j) the 5-HT7 receptor knocked down in P1 neurons. The mean ∆F/F0 (%) comparing the before and after application of 5-HT time windows (n = 7 and 7) is also shown (right). k, plP10 Sp-GAL4 driving UAS-mCD8-GFP (green) in the adult brain. Anti-nC82 is in magenta. Scale bar, 50 μm. l, plP10 > 5-HT2B-RNAi;Dicer males respond less to the threat (n = 18, 20 and 24 (no threat); n = 11, 15 and 20 (threat)). m,n, ∆F/F0 (%) of the P1 > GCaMP6m signal pre-application and post-application of 100 µM 5-HT without (m) or with (n) the 5-HT2B receptor knocked down in p1P10 neurons. The mean ∆F/F0 (%) comparing the before and after application of 5-HT time windows (n = 6 and 6) is also shown (right). o, Network model. The dashed lines indicate non-functionally established connections. CPG, central pattern generator. Refer to the legend of Fig. 1 for details on graphics and statistics.
