Extended Data Fig. 3: Visually-driven 5-HTPMPD neurons inhibit the P1 central courtship node via 5-HT7- Gαi signalling.
A, Courtship index of P1>CsChrimson males without (grey bars, n = 27,31,25) or with (blue bars, n = 34,31,35) the threat (red light OFF). B, ∆F/F0% of TRHPMPD > GCaMP6m signal upon laser stimulation. Left: mean ∆F/F0% signal comparing baseline to stimulation (n = 9). Right: focal plane showing ROI during imaging (scale bar: 15 µm) refer to Fig. 2e. C, Change in walking speed of TRHR23E12 > TNT males and controls before and after threat (n = 31,33,40). D, Defensive index of TRHR23E12 > TNT males and controls without (grey bars, n = 15,15,17) or with the threat (blue bars, n = 14,19,24). E,F, Courtship and defensive indexes of TRH > TRH-RNAi males and controls without (grey plots, n = 18,21,25) or with threat (blue plots, n = 18,23,25). G, Defensive index of TRHR23E12>CsChrimson males upon artificial activation without threat (nred light OFF = 17,19,25; nred light ON = 14, 22, 19) and controls. H, Behavioural effects of knocking-down 5-HT receptors in P1 neurons using RNAi, in control (n = 38) and experimental males (n = 18,15,12,29). I, Behavioural effects of knocking-down Gαi and Gαo proteins in P1 neurons using RNAi (n = 9,12,15), and controls (n = 17). J, Upper: ∆F/F0% of P1 > GCaMP6s signal pre and post application of 100 µM 5-HT and with simultaneous knock-down of Gαi protein (lower). Right: mean ∆F/F0% comparing the pre and post time windows (n = 6,7). K, Change in walking speed of P1 > 5-HT7-RNAi and P1>Gαi-RNAi males before and after threat (n = 58,64,70). See legend to Extended Data Fig. 1 for details on graphics and statistics.
