Extended Data Fig. 5: Validation of GR as import shuttle for FKBP12F36V.
From: Targeted protein relocalization via protein transport coupling
a. TRAM engaging GR and FKBP12F36V. b, Representative live-cell images of GR dependent FKBP12F36V import upon treatment with 4 or unlinked warheads 8 and 3 for 3 h. c, FKBP12F36V nuclear import dose response derived from the analysis pipeline after a 3-hour treatment with 4 engaging GR. d, Nuclear localization of mCherry-FKBP12F36V after treatment with 4 or unlinked warheads 3 and 8 for 3 h. e, Nuclear localization of GR after treatment with 4 or unlinked warheads 3 and 8 for 3 h. f, Representative live-cell images of the unlinked warhead competition assay for GR-dependent FKBP12F36V import after 3-hour treatment. g, mCherry localization upon treatment with 4 in competition with either 1x or 10x of 8 or 3 for 3 h. h, Nuclear localization of GR upon treatment with 4 in competition with either 1x or 10x of 8 or 3 for 3 h. Images in b, f, are representative of three biological replicates. Data in c, d, e, g, h are mean ± s.d. of three independent experiments. Scale bars are 20 µm. P values in c, d, e, g, h were determined by one-way ANOVA with Dunnett’s post hoc test comparing each condition to the DMSO control. Hoechst stains the nucleus.
