Extended Data Fig. 11: Validation of Cas9-CRISPR mediated endogenously tagged lines.
From: Targeted protein relocalization via protein transport coupling
a, Schematic for modified PITCh knock-in into target proteins. b, Sequencing of the regions near the N terminus of the endogenous PRMT9 gene after donor cassette insertion into endogenous PRMT9. c, Sequencing of the region downstream the N terminus of the endogenous SOS1 gene after donor cassette insertion into endogenous SOS1. d, Due to high GC content of the genomic region upstream of the N terminus of SOS1, immunoblotting was used to validate successful incorporation of donor cassette onto endogenous SOS1 after GFP pulldown, I = input, F = flowthrough, E = eluate. e, Sequencing of the regions near the N terminus of the endogenous FKBP12 gene after donor cassette insertion into endogenous FKBP12. f, Demonstration of GFP-FKBP12F36V tagging of endogenous PRMT9, SOS1 and GFP tagging of endogenous FKBP12 in HEK293T cells. Immunoblots in d,f are representative of two independent experiments. Uncropped blots can be found in Supplementary Fig. 7.
